Long-Term Survival of Streptococcus pneumoniae at Room Temperature on Dorset Egg Medium

Author:

Wasas Avril D.1,Huebner Robin E.1,De Blanche Mignon1,Klugman Keith P.1

Affiliation:

1. MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg, South Africa

Abstract

ABSTRACT Forty-five isolates of Streptococcus pneumoniae were inoculated on Dorset egg and supplemented Columbia agar base media, incubated overnight at 37°C, and then kept at room temperature (RT; 21°C) or 4°C. Long-term viability was best at RT for both media, with all isolates remaining viable on Dorset egg medium for 44 days; viability was 90 and 57% on Columbia agar base medium after 7 and 30 days. We recommend the use of Dorset egg medium for the maintenance of pneumococci at RT.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

1. De Blanche M. A. Wasas and K. Klugman. Unpublished data.

2. Difco Laboratories Difco Manual 10th ed. 1984 Difco Laboratories Detroit Mich

3. The use of eggs as a medium for the cultivation of Bacillus tuberculosis;Dorset M.;Am. Med.,1902

4. The magnitude of mortality from acute respiratory infections in children under 5 years in developing countries;Garenne M.;World Health Stat. Q.,1992

5. Development of CABS, a storage and transport medium for Haemophilus influenzae and Streptococcus pneumoniae;Gratten M.;Austral. Microbiol.,1996

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