Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803-Reference-Cited by-同舟云学术

Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803

Published:2004-12 Issue:23 Volume:186 Page:8156-8158
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Barkley Sam J.¹,Desai Shrivallabh B.¹,Poulter C. Dale¹

Affiliation:

1. Department of Chemistry, University of Utah, Salt Lake City, Utah

Abstract

ABSTRACT Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His 6 -tagged protein was produced in Escherichia coli and purified by Ni 2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg 2+ for activity; K m IPP was 52 μM, and k cat IPP was 0.23 s −1 .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.186.23.8156-8158.2004

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