The Aer Protein of Escherichia coli Forms a Homodimer Independent of the Signaling Domain and Flavin Adenine Dinucleotide Binding

Author:

Ma Qinhong1,Roy Francis1,Herrmann Sarah1,Taylor Barry L.1,Johnson Mark S.1

Affiliation:

1. Division of Microbiology and Molecular Genetics, Loma Linda University, Loma Linda, California

Abstract

ABSTRACT In vivo cross-linking between native cysteines in the Aer receptor of Escherichia coli showed dimer formation at the membrane anchor and in the putative HAMP domain. Dimers also formed in mutants that did not bind flavin adenine dinucleotide and in truncated peptides without a signaling domain and part of the HAMP domain.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

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