Affiliation:
1. Institut für Biochemie der Universität zu Köln, Cologne, Germany
Abstract
ABSTRACT
The molecular identification of the
Corynebacterium glutamicum
urea uptake system is described. This ABC-type transporter is encoded by the
urtABCDE
operon, which is transcribed in response to nitrogen limitation. Expression of the
urt
genes is regulated by the global nitrogen regulator AmtR, and an
amtR
deletion strain showed constitutive expression of the
urtABCDE
genes. The AmtR repressor protein also controls transcription of the urease-encoding
ureABCEFGD
genes in
C. glutamicum
. The
ure
gene cluster forms an operon which is mainly transcribed in response to nitrogen starvation. To confirm the increased synthesis of urease subunits under nitrogen limitation, proteome analyses of cytoplasmic protein extracts from cells grown under nitrogen surplus and nitrogen limitation were carried out, and five of the seven urease subunits were identified.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
74 articles.
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