Purification of Fully Activated Clostridium botulinum Serotype B Toxin for Treatment of Patients with Dystonia-Reference-Cited by-同舟云学术

Purification of Fully Activated Clostridium botulinum Serotype B Toxin for Treatment of Patients with Dystonia

Published:2003-03 Issue:3 Volume:71 Page:1599-1603
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Arimitsu Hideyuki¹,Inoue Kaoru¹,Sakaguchi Yoshihiko¹,Lee Jaechul¹,Fujinaga Yukako¹,Watanabe Toshihiro²,Ohyama Tohru²,Hirst Robert³,Oguma Keiji¹

Affiliation:

1. Department of Bacteriology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558

2. Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri 099-2493, Japan

3. Department of Microbiology and Immunology, James Cook University, Townsville 4811, Australia

Abstract

ABSTRACT Clostridium botulinum serotype B toxins 12S and 16S were separated by using a beta-lactose gel column at pH 6.0; toxin 12S passed through the column, whereas toxin 16S bound to the column and eluted with lactose. The fully activated neurotoxin was obtained by applying the trypsin-treated 16S toxin on the same column at pH 8.0; the neurotoxin passed through the column, whereas remaining nontoxic components bound to the column. The toxicity of this purified fully activated neurotoxin was retained for a long period by addition of albumin in the preparation.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.71.3.1599-1603.2003

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