CHARACTERISTICS OF PARA-INFLUENZA 1 (HA-2) VIRUS III

Author:

Dick Elliot C.1,Mogabgab William J.1

Affiliation:

1. Department of Medicine, Tulane University School of Medicine, New Orleans, Louisiana

Abstract

Dick, Elliot C. (Tulane University School of Medicine, New Orleans, La.) and William J. Mogabgab . Characteristics of para-influenza 1 (HA-2) virus. III. Antigenic relationships, growth, interaction with erythrocytes, and physical properties. J. Bacteriol. 83: 561–571. 1962—Hemagglutination-inhibition tests with rabbit and rooster immune sera showed HA-2-like strains of para-influenza 1 to be nearly as closely related antigenically to para-influenza 3 (HA-1) virus as to the Sendai strain of para-influenza 1; however, convalescent sera from humans infected with para influenza 1 virus exhibited four-fold or greater increases in antibody to HA-2 and Sendai viruses, but seldom to HA-1. Para-influenza 1 (HA-2) infectivity was destroyed within 2 hr at temperatures approaching 50 C; at 36 C, infective virus disappeared by 80 hr. At a temperature of 44 C or below, the hemagglutinin (HA) titer remained unchanged for 100 hr; but at temperatures above this, hemagglutinin was destroyed rapidly. Virus infectivity was found to be most stable at very slightly acid or neutral pH and to be increasingly labile at pH values more extreme than 5.7 and 8.7. Hemagglutinin was generally more resistant than infectivity to extremes of reaction; this was most notable in the alkaline range. In primary monkey-kidney tissue culture, virus was adsorbed in 5 hr, new infective virus was noted in 11 to 15 hr, and maximal amounts were detected at 60 hr and maintained through 132 hr. Production of hemagglutinin paralleled that of infectivity. The tcid 50 /HA ratio was approximately 4.5. With human type “O” erythrocytes, the HA titer of Copenhagen 222 virus suspensions was raised two- to fourfold by increasing the temperature of reaction and sedimentation from 4 C to room temperature. It could not be demonstrated that this thermal effect was due either to an increased total adsorption at an elevated temperature or to a greater rate of adsorption; the rate of hemagglutination was, however, increased by fourfold at the higher temperature.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference20 articles.

1. Para-influenza viruses 1, 2, and 3: suggested names for recently described myxoviruses;ANDREWES C. H.;Virology,1959

2. The mechanism of hemagglutination by influenza virus;BUZZELL A.;Advances in Virus Research,1958

3. Newly recognized myxoviruses from children with respiratory disease;CHANOCK R. M.;New Engl. J. Med.,1958

4. Association of hemadsorption viruses with respiratory illness in childhood;CHANOCK R. M.;J. Am. Med. Assoc.,1959

5. COLOBERT L. AND R. FONTANGES. 1959. Titrage de l'activite hemagglutinante et de l'activite enzymatique de Myxovirus para-influenzae 1 (virus Sendai). Compt. rend. soc. biol. 153: 1382-1386.

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