Modified micromethod of the Limulus amoebocyte lysate assay for endotoxin

Author:

Melvaer K L,Fystro D

Abstract

A Limulus amoebocyte lysate microtechnique performed in petrolatum wells on a microscope slide is described. Injection of a dye solution in ethanol directly into the wells leads to an unambiguous interpretation of the results. Twelve samples can be tested on a single slide, and compact storing of the samples is possible.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference11 articles.

1. A microtechnique for endotoxin assay by using Limulus Iysate;Flowers D. J.;Med. Lab. Sci.,1979

2. Slide test as a micromethod of a modified Limulus endotoxin test;Frauch P.;J. Pharm. Sci.,1974

3. Improved microtechnique for endotoxin assay by the Limulus amebocyte lysate test;Gardi A.;Anal. Biochem.,1980

4. Dry up method as a revised Limulus test with a new technique for gelation inhibitor removing;Goto H.;Jpn. J. Exp. Med.,1979

5. Harada T. T. Morita S. Twanaga S. Nakamura and M. Niwa. 1978. Assay of bacterial endotoxins using Limulus hemocyte Iysate p. 209-220. In E. Cohen (ed.) Biomedical applications of the horseshoe crab (Limulidae). Alan R. Liss Inc. New York.

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