EBNA-LP Associates with Cellular Proteins Including DNA-PK and HA95-Reference-Cited by-同舟云学术

EBNA-LP Associates with Cellular Proteins Including DNA-PK and HA95

Published:2001-03 Issue:5 Volume:75 Page:2475-2481
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Han Innoc¹,Harada Shizuko¹,Weaver David²,Xue Yong¹,Lane William³,Orstavik Sigurd⁴,Skalhegg Bjorn⁴,Kieff Elliott¹

Affiliation:

1. Channing Laboratory, Harvard Medical School, Boston,1 and

2. Center for Blood Research, Harvard University,2 and

3. Harvard Microchemistry Facility,3 Cambridge, Massachusetts, and

4. Institute of Medical Biochemistry, University of Oslo, Blindern, N-0317 Oslo, Norway4

Abstract

ABSTRACT EBNA-LP-associated proteins were identified by sequencing proteins that immunoprecipitated with Flag epitope-tagged EBNA-LP (FLP) from lymphoblasts in which FLP was stably expressed. The association of EBNA-LP with Hsp70 (72/73) was confirmed, and sequences of DNA-PK catalytic subunit (DNA-PKcs), HA95, Hsp27, prolyl 4-hydroxylase α-1 subunit, α-tubulin, and β-tubulin were identified. The fraction of total cellular HA95 that associated with FLP was very high, while progressively lower fractions of the total DNA-PKcs, Hsp70, Hsp 27, α-tubulin, and β-tubulin specifically associated with EBNA-LP as determined by immunoblotting with antibodies to these proteins. EBNA-LP bound to two domains in the DNA-PKcs C terminus and DNA-PKcs associated with the EBNA-LP repeat domain. DNA-PKcs that was bound to EBNA-LP phosphorylated p53 or EBNA-LP in vitro, and the phosphorylation of EBNA-LP was inhibited by Wortmannin, a specific in vitro inhibitor of DNA-PKcs.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.75.5.2475-2481.2001

Reference78 articles.

1. Early events in Epstein-Barr virus infection of human B lymphocytes;Alfieri C.;Virology,1991

2. Epstein-Barr virus nuclear antigen EBNA3C/6 expression maintains the level of latent membrane protein 1 in G1-arrested cells

3. DNA double-strand break repair proteins are required to cap the ends of mammalian chromosomes;Bailey S. M.;Proc. Natl. Acad. Sci. USA,1999

4. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation;Blunt T.;Cell,1995

5. A DNA-activated protein kinase from HeLa cell nuclei

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