Affiliation:
1. Biomacromolecules Research Laboratory, Department of Microbiology, Gyeongsang National University, Chinju 660-701, Republic of Korea
Abstract
Forty-two different carbon sources were tested for the polyester synthesis of a citronellol-utilizing bacterium,
Pseudomonas citronellolis
(ATCC 13674). These included linear C
2
to C
10
monocarboxylic acids, C
3
to C
10
dicarboxylic acids, saccharides, α,ω-diols, hydrocarbons, and 3-methyl-branched substrates such as 3,7-dimethyl-6-octen-1-ol (citronellol), 3-methyl-
n
-valerate, 3-methyl-1-butanol, and 3-methyladipate. Isolated polymers were characterized by gas chromatography, infrared spectroscopy,
1
H- or
13
C-nuclear magnetic resonance spectroscopy,
1
H-
13
C heteronuclear correlation spectroscopy (
1
H-
13
C COSY),
1
H-
1
H homonuclear COSY, and differential scanning calorimetry. Polyesters from nine monocarboxylic acids and two related carbon sources could be metabolically divided into three groups. The first group of C
2
to C
4
carbon sources resulted in copolyesters composed of 61 to 70 mol% 3-hydroxydecanoate, 23 to 33 mol% 3-hydroxyoctanoate, 3.6 to 9.0 mol% 3-hydroxy-5-
cis
-dodecenoate, and 1.8 to 2.6 mol% 3-hydroxy-7-
cis
-tetradecenoate. Carbon sources in group II (C
7
to C
10
) produced copolyesters composed of 3-hydroxyacid monomer units with the same number of carbon atoms as the substrate (major constituent) and monomer units with either two less or two more carbons. Negligible amounts of 3-hydroxy-5-
cis
-dodecenoate and 3-hydroxy-7-
cis
-tetradecenoate were detected in copolyesters from this group. Copolyesters from group III (C
5
and C
6
) had a monomer unit distribution that could be said to be between those of groups I and II. In addition, a novel copolyester, poly(3-hydroxy-7-methyl-6-octenoate-co-3-hydroxy-5-methylhexanoate), was synthesized when grown on citronellol. The
1
H-
13
C heteronuclear COSY spectrum for monomer unit II revealed that both methylene and isopropyl groups, proximately connected in series to a single chiral center, had magnetically diastereotopic natures.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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