Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density Plasmodium falciparum Infection from Controlled Human Malaria Infection Studies and Naturally Acquired Infections-Reference-Cited by-同舟云学术

Recombinase Polymerase Amplification and Lateral Flow Assay for Ultrasensitive Detection of Low-Density Plasmodium falciparum Infection from Controlled Human Malaria Infection Studies and Naturally Acquired Infections

Published:2020-04-23 Issue:5 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lalremruata Albert¹,Nguyen The Trong¹²,McCall Matthew B. B.¹³,Mombo-Ngoma Ghyslain¹³,Agnandji Selidji T.¹³,Adegnika Ayôla A.¹³⁴,Lell Bertrand¹³,Ramharter Michael³⁵⁶,Hoffman Stephen L.⁷,Kremsner Peter G.¹³,Mordmüller Benjamin¹³

Affiliation:

1. Institut für Tropenmedizin, Universität Tübingen and German Center for Infection Research, Tübingen, Germany

2. Vietnamese—German Center of Excellence in Medical Research, Hanoi, Vietnam

3. Centre de Recherches Médicales de Lambaréné and African Partner Institution, German Center for Infection Research, Lambaréné, Gabon

4. Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands

5. Department of Tropical Medicine, Bernhard Nocht Institute for Tropical Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

6. Department of Medicine, University Medical Center Hamburg-Eppendorf, Hamburg, Germany

7. Sanaria Inc., Rockville, Maryland, USA

Abstract

Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemias that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed. We compared the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples ( n = 114) obtained after standardized controlled human malaria infection (CHMI) with Plasmodium falciparum sporozoites.

Funder

HHS | National Institutes of Health

Deutsches Zentrum für Infektionsforschung

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01879-19

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