A Versatile Reporter System To Monitor Virus-Infected Cells and Its Application to Dengue Virus and SARS-CoV-2-Reference-Cited by-同舟云学术

A Versatile Reporter System To Monitor Virus-Infected Cells and Its Application to Dengue Virus and SARS-CoV-2

Published:2021-01-28 Issue:4 Volume:95 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Pahmeier Felix¹^ORCID,Neufeldt Christopher J.¹,Cerikan Berati¹,Prasad Vibhu¹^ORCID,Pape Costantin²³,Laketa Vibor⁴,Ruggieri Alessia¹,Bartenschlager Ralf¹⁴⁵^ORCID,Cortese Mirko¹^ORCID

Affiliation:

1. Department of Infectious Diseases, Molecular Virology, University of Heidelberg, Heidelberg, Germany

2. HCI/IWR, Heidelberg University, Heidelberg, Germany

3. European Molecular Biology Laboratory, Heidelberg, Germany

4. German Center for Infection Research, Heidelberg partner site, Heidelberg, Germany

5. Division “Virus-Associated Carcinogenesis”, German Cancer Research Center (DKFZ), Heidelberg, Germany

Abstract

Reporter systems are useful tools for fast and quantitative visualization of virus-infected cells within a host cell population. Here, we describe a reporter system that takes advantage of virus-encoded proteases expressed in infected cells to cleave an ER-anchored fluorescent protein fused to a nuclear localization sequence.

Funder

Deutsche Forschungsgemeinschaft

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.01715-20

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