SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication-Reference-Cited by-同舟云学术

SARS-CoV-2 N Protein Antagonizes Stress Granule Assembly and IFN Production by Interacting with G3BPs to Facilitate Viral Replication

Published:2022-06-22 Issue:12 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Liu Hainan¹^ORCID,Bai Yu²,Zhang Xun²,Gao Ting¹,Liu Yue¹,Li Entao³,Wang Xuefeng³^ORCID,Cao Zheng⁴,Zhu Lin¹,Dong Qincai¹,Hu Yong¹,Wang Guangfei¹,Song Caiwei¹,Niu Xiayang²,Zheng Tong¹,Wang Di²,Liu Zijing¹,Jin Yanwen¹,Li Ping¹,Bian Xiuwu⁵,Cao Cheng¹,Liu Xuan¹^ORCID

Affiliation:

1. Beijing Institute of Biotechnology, Beijing, China

2. Anhui University, Hefei, China

3. Changchun Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, Jilin, China

4. Hubei University of Technology, Wuhan, China

5. First Affiliated Hospital, Army Medical University, Chongqing, China

Abstract

In this study, by in vitro assay and live SARS-CoV-2 virus infection, we provide solid evidence that the SARS-CoV-2 NP associates with G3BP1 and G3BP2 in vitro and in vivo . NP SARS-CoV-2 could efficiently suppress G3BP-mediated SG formation and potentiate viral infection by overcoming antiviral innate immunity mediated by G3BP1 in A549 cell lines and G3BP1 conditional knockout mice ( g3bp1 -cKO) mice, which provide in-depth evidence showing the mechanism underlying NP-related SARS-CoV-2 pathogenesis through G3BPs.

Funder

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00412-22

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