Abstract
The immune response of mice to live, heat-killed, or autoclaved Mycobacterium leprae was investigated. After sensitization with 10(7) organisms in each group, recipient mice were transfused with the sensitized splenocytes 28 days later. A selected number of these mice were infected with 5 X 10(3) M. leprae, and the remaining animals were sacrificed at scheduled intervals for evidence of cell-mediated immunity to the M. leprae cell extract. Data from these and the bacteriological assays showed that all three materials induce cell-mediated immunity and also extend protection against the M. leprae challenge but not against a Listeria monocytogenes challenge. Adoptive immunity against M. leprae was expressed equally effectively in both non-irradiated animals and those sublethally (500 R) irradiated. This study reveals that, after adoptive transfer of immunity, a bacillary restriction occurs with concomitant onset of delayed hypersensitivity and that the protection observed could be specifically directed against an M. leprae challenge.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference15 articles.
1. Contact sensitivity in the mouse. IV. The role of Iymphocytes and macrophages in passive transfer and the mechanism of their interaction;Asheron G. L.;J. Exp. Med.,1970
2. Bruning J. L. and B. L. Kintz. 1977. Computation handbook of statistics 2nd ed. Scott Foresman and Co. Glenview Ill.
3. Quantitative studies of the adoptive immunological memory of mice. 1. An age dependent barrier to syngeneic transplantation;Celada F.;J. Exp. Med.,1966
4. The cellular basis of immunologic memory;Celada F.;Prog. Allergy,1971
5. Evidence of suppressor cell activity in spleens of mice bearing primary tumors induced by Maloney sarcoma virus;Kirchner H.;J. Exp. Med.,1974
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