Affiliation:
1. Microbiology Section, Department of Biological Sciences, University of Southern California, Los Angeles, California
Abstract
Bartholomew
, J. W. (University of Southern California, Los Angeles),
and Thomas Cromwell
. Relative contribution of the cell wall, cytoplasmic membrane, and cytoplasm to the gram-positive characteristic of
Bacillus megaterium
. J. Bacteriol.
90:
643–647. 1965.—A comparison of the roles of the cell wall, cytoplasmic membrane, and cytoplasmic components revealed that the intact cell wall was the dominant contributor to the gram-positive state. Protoplasts of
Bacillus megaterium
were confirmed as being gram-negative, as reported by Gerhardt et al. The “gram-positive protoplast” report of Amano et al. was shown to be a laboratory-produced artifact, resulting from the comparison of smears made from saline suspensions of
Escherichia coli
cells with smears made from formalin-sucrose suspensions of
B. megaterium
protoplasts.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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