Affiliation:
1. McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland
Abstract
Duckworth, Donna
H. (Johns Hopkins University, Baltimore, Md.),
and Maurice J. Bessman
. Assay for the killing properties of T2 bacteriophage and their “ghosts.” J. Bacteriol.
90:
724–728. 1965.—A procedure for the assay of bacteriophage and their “ghosts” which is based on their ability to kill cells is described. The method is derived from the well-known ability of phage and ghosts to prevent the induction of β-galactosidase. Conditions are described whereby a direct relationship is found between the decrease in β-galactosidase and the number of phage or ghosts present during the induction period. The number of phage measured by this method was found to be identical with the number of plaque-forming units found in a fresh lysate. The method has been used to follow the fate of ghosts under several conditions and to measure killer (but nonviable) particles in various preparations of phage.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference16 articles.
1. Bacterial viruses;ADAMS M. H.;Methods Med. Res.,1950
2. Destruction of bacterial viruses by osmotic shock;ANDERSON T. F.;J. Appl. Phys.,1950
3. Induced synthesis of enzymes in bacteria analyzed at the cellular level;BENZER S.;Biochim. Biophys. Acta,1953
4. Etude de l'action des membranes du bacteriophage T2 sur Escherichia coli;BONIFAS V.;Biochim. Biophys. Acta,1955
5. The action of T2 bacteriophage ghosts on Escherichia coli B;FRENCH R. C.;Can. J. Microbiol.,1955
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