Affiliation:
1. Department of Medicine, Stanford University, Stanford, California 94305
Abstract
Experiments were done to show that the human hepatitis B antigen (HBAg)-associated DNA polymerase is a component of Dane particles and their antigenically distinct cores prepared by Nonidet P-40 detergent treatment of Dane particles. Before detergent treatment, the DNA polymerase was precipitated by serum containing anti-HB surface antigen (anti-HB
s
) but not with serum containing anti-HB core antigen (anti-HB
c
). After detergent treatment, the enzyme was precipitated by anti-HB
c
- and not by anti-HB
s
-containing serum. Highly purified 16- to 25-nm HBAg particles blocked only the precipitation of DNA polymerase in untreated HBAg preparations. The 110
S
structure with which the DNA reaction product remains associated in Nonidet P-40-treated preparations was identified as Dane particle core by immunoprecipitation with serum containing anti-HB
c
. The DNA polymerase and the radioactive DNA reaction product were used as markers for core in immunoprecipitation tests for anticore. In such assays, 8 of 11 human sera with anti-HB
s
activity and all of 10 sera from chronic HBAg carriers were found to contain anti-HB
c
activity.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
146 articles.
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