Affiliation:
1. Program in Molecular and Cellular Biology, Ohio University, Athens 45701.
Abstract
Recombinant plasmids pMTIF-beta 1A and pMTIF-beta 1B were constructed by fusing the metallothionein I promoter-regulatory region to the human beta 1 interferon (HuIFN-beta 1) gene. These linearized fusion genes were then introduced into mouse germ lines by zygote microinjection. The chromosomal integration and the germ line transmission of the injected DNA sequences in the resulting transgenic mice were detected by DNA dot blot and Southern transfer hybridizations. The sera of at least two strains of metallothionein/HuIFN transgenic mice were found to protect human WISH cells against vesicular stomatitis virus infection, and this activity could be neutralized by preincubation with anti-HuIFN-beta 1 antibody. These transgenic mice demonstrated significantly enhanced resistance to pseudorabies virus compared with nontransgenic mice when inoculated with pseudorabies virus. The level of resistance seemed to correlate with the concentrations of HuIFN-beta 1 in serum. These transgenic mice may be used as models to study IFN-induced responses and may serve as prototypes to generate disease-resistant animals.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference17 articles.
1. Brinster R. L. H. Y. Chen M. Trumbauer A. W. Senear R. Warren and R. D. Palmiter. 1981. Somatic expression of herpes thymidine kinase in mice following injection of a fusion gene
2. Expression of human fibroblast interferon gene in E. coli;Derynck R.;Nature (London),1980
3. Species-specificity of interferon;Gillespie G.;Tex. Rep. Biol. Med.,1981
4. Expression of interferon in the testes of transgenic mice leads to infertility;Hekman R. A. C. P.;J. Interferon Res.,1987
5. Male transgenic mice carrying extra interferon gene are unable to transmit the exogenous gene to their offspring;Iwakura Y.;J. Interferon Res.,1987
Cited by
15 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献