Long-Term Culture of Giardia lamblia in Cell Culture Medium Requires Intimate Association with Viable Mammalian Cells-Reference-Cited by-同舟云学术

Long-Term Culture of Giardia lamblia in Cell Culture Medium Requires Intimate Association with Viable Mammalian Cells

Published:2019-11 Issue:11 Volume:87 Page:
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Nash Theodore E.¹

Affiliation:

1. Laboratory of Parasitic Diseases, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland, USA

Abstract

Giardia lamblia is usually cultured axenically in TYI-S-33, a complex medium which does not permit survival and growth of mammalian cells. Likewise, medium commonly used to maintain and grow mammalian cells does not support healthy trophozoite survival for more than a few hours. The inability to coculture trophozoites and epithelial cells under optimal conditions limits studies of their interactions as well as interpretation of results.

Funder

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.00639-19

Reference12 articles.

1. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile

2. Attachment of the flagellate Giardia lamblia: role of reducing agents, serum, temperature, and ionic composition.

3. Restriction-Endonuclease Analysis of DNA from 15 Giardia Isolates Obtained from Humans and Animals