Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods

Author:

Tolpinrud Anita1ORCID,Stenos John2,Chaber Anne-Lise3,Devlin Joanne M.1,Herbert Catherine4,Pas An5,Dunowska Magdalena6,Stevenson Mark A.1,Firestone Simon M.1ORCID

Affiliation:

1. Asia Pacific Centre for Animal Health, Melbourne Veterinary School, The University of Melbourne, Parkville, Victoria, Australia

2. Australian Rickettsial Reference Laboratory, University Hospital Geelong, Geelong, Victoria, Australia

3. School of Animal and Veterinary Sciences, The University of Adelaide, Roseworthy, South Australia, Australia

4. School of Life and Environmental Sciences, The University of Sydney, Camperdown, New South Wales, Australia

5. New Zealand Centre for Conservation Medicine, Auckland Zoo, Auckland, New Zealand

6. School of Veterinary Science, Massey University, Palmerston North, New Zealand

Abstract

Kangaroos are considered to be an important reservoir of Q fever in Australia, although there is limited knowledge on the true prevalence and distribution of coxiellosis in Australian macropod populations. Serological tests serve as useful surveillance tools, but formal test validation is needed to be able to estimate true seroprevalence rates, and few tests have been validated to screen wildlife species for Q fever.

Funder

Department of Agriculture, Water and the Environment

Cybec Foundation

Department of Education, Skills and Employment, Australian Government

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference58 articles.

1. From Q Fever to Coxiella burnetii Infection: a Paradigm Change

2. “Q” FEVER, A NEW FEVER ENTITY: CLINICAL FEATURES, DIAGNOSIS AND LABORATORY INVESTIGATION

3. World Organisation for Animal Health. 2018. Chapter 3.1.16: Q fever. In Manual of diagnostic tests and vaccines for terrestrial animals, 8th ed. World Organisation for Animal Health, Paris, France. https://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/3.01.16_Q_FEVER.pdf.

4. Coxiellosis in reptiles of South Kanara district;Stephen S;Indian J Med Res,1979

5. POIKILOTHERMS AS RESERVOIRS OF Q-FEVER (Coxiella burnetii) IN UTTAR PRADESH

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