Identification of Mycobacterial Antigens in Human Urine by Use of Immunoglobulin G Isolated from Sera of Patients with Active Pulmonary Tuberculosis-Reference-Cited by-同舟云学术

Identification of Mycobacterial Antigens in Human Urine by Use of Immunoglobulin G Isolated from Sera of Patients with Active Pulmonary Tuberculosis

Published:2016-06 Issue:6 Volume:54 Page:1631-1637
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Kim Sun Hee¹,Lee Nan-Ee¹,Lee Jong Seok²,Shin Jeong Hwan³,Lee Ju Yeon⁴,Ko Jeong-Heon¹⁵,Chang Chulhun Ludgerus⁶,Kim Yong-Sam¹⁵

Affiliation:

1. Genome Editing Research Center, KRIBB, Deajeon, South Korea

2. International Tuberculosis Research Center, Changwon-si, South Korea

3. Department of Laboratory Medicine and Paik Institute for Clinical Research, Inje University College of Medicine, Busan, South Korea

4. Division of Mass Spectrometry, Korea Basic Science Institute, Cheongwon-Gun, South Korea

5. Korea University of Science and Technology, Daejeon, South Korea

6. Department of Laboratory Medicine, School of Medicine, Pusan National University, Busan, South Korea

Abstract

ABSTRACT Point-of-care (POC) diagnostic testing of tuberculosis (TB) is a tremendous unmet need. In this study, four urinary mycobacterial antigens were identified through two independent approaches using IgG capture and immunodepletion methods. Among these, ModC was validated by a multiple reaction monitoring (MRM) method. As expected, the biomarkers elevated the clinical validity of TB diagnosis when combined with preexisting markers.

Funder

Ministry of Health and Welfare

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00236-16

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