Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters-Reference-Cited by-同舟云学术

Construction of broad-host-range plasmid vectors for easy visible selection and analysis of promoters

Published:1990-06 Issue:6 Volume:172 Page:3496-3499
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Farinha M A¹,Kropinski A M¹

Affiliation:

1. Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.

Abstract

We have constructed a series of broad-host-range plasmids which use "visual screens" to detect promoter activity. These plasmids contain the pMB1 and pRO1600 origins of replication and are capable of replicating in a wide range of gram-negative bacteria. The genes encoding beta-galactosidase and alkaline phosphatase from Escherichia coli and bacterial luciferase from Vibrio harveyi supply the promoterless indicator genes. The constructs were tested in E. coli and Pseudomonas aeruginosa.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.172.6.3496-3499.1990

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