Transfer region of IncI1 plasmid R64 and role of shufflon in R64 transfer

Author:

Komano T1,Funayama N1,Kim S R1,Nisioka T1

Affiliation:

1. Department of Biology, Tokyo Metropolitan University, Japan.

Abstract

To locate the transfer region of the 122-kiloase plasmid R64drd-11 belonging to incompatibility group I1, a series of deletion derivatives was constructed by in vitro recombinant DNA techniques followed by double homologous recombination in vivo. A plasmid designated pKK609 and bearing a 56.7-kilobase R64 sequence was the smallest transferable plasmid. A plasmid designated pKK610 and no longer possessing the 44-base-pair sequence of the R64 transfer system is located at one end. The other end of the R64 transfer region comprises a DNA segment of about 19 kilobases responsible for pilus formation. Shufflon, DNA with a novel rearrangement in R64, was found to be involved in pilus formation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference26 articles.

1. Derepressed plasmids of incompatibility group I determine two different morphological forms of pilus;Bradley D. E.;Plasmid,1983

2. Characteristics and function of thick and thin conjugative pili determined by transfer-derepressed plasmids of incompatibility groups 11, 12, Is, B;Bradley D. E.;K and Z. J. Gen. Microbiol.,1984

3. Analysis of gene control signal by DNA fusion and cloning in Escherichia coli;Casadaban M. J.;J. Mol. Biol.,1980

4. Phages Ia and 12-2: IncI plasmid-dependent bacteriophages;Coetzee J. N.;J. Gen. Microbiol.,1982

5. Properties of a ifiamentous phage which adsorbs to pili coded by plasmids of the IncI complex;Coetzee J. N.;J. Gen. Microbiol.,1980

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