Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1-Reference-Cited by-同舟云学术

Characterization of a Class II Defective Transposon Carrying Two Haloacetate Dehalogenase Genes from Delftia acidovorans Plasmid pUO1

Published:2002-05 Issue:5 Volume:68 Page:2307-2315
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Sota Masahiro¹²,Endo Masahiro¹,Nitta Keiji¹,Kawasaki Haruhiko³,Tsuda Masataka²

Affiliation:

1. Department of Environmental Simulation, Institute for Environmental Sciences, Obuchi, Rokkasho, Aomori 039-3212

2. Department of Environmental Life Sciences, Graduate School of Life Sciences, Tohoku University, Sendai 980-8577

3. Department of Applied Biochemistry, Graduate School of Agriculture and Biological Science, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan

Abstract

ABSTRACT The two haloacetate dehalogenase genes, dehH1 and dehH2 , on the 65-kb plasmid pUO1 from Delftia acidovorans strain B were found to be located on transposable elements. The dehH2 gene was carried on an 8.9-kb class I composite transposon (Tn Had1 ) that was flanked by two directly repeated copies of IS 1071 , IS 1071 L and IS 1071 R. The dehH1 gene was also flanked by IS 1071 L and a truncated version of IS 1071 (IS 1071 N). Tn Had1 , dehH1 , and IS 1071 N were located on a 15.6-kb class II transposon (Tn Had2 ) whose terminal inverted repeats and res site showed high homology with those of the Tn 21 -related transposons. Tn Had2 was defective in transposition because of its lacking the transposase and resolvase genes. Tn Had2 could transpose when the Tn 21 -encoded transposase and resolvase were supplied in trans . These results demonstrated that Tn Had2 is a defective Tn 21 -related transposon carrying another class I catabolic transposon.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.5.2307-2315.2002

Reference52 articles.

1. Allmeier, H., B. Cresnar, M. Greck, and R. Schmitt. 1992. Complete nucleotide sequence of Tn1721: gene organization and a novel gene product with features of a chemotaxis protein. Gene111:11-20.

2. Amemura-Maekawa, J., and E. Ohtsubo. 1991. Functional analysis of the two domains in the terminal inverted repeat sequence required for transposition of Tn3. Gene103:11-16.

3. Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Struhl. 1994. Current protocols in molecular biology. Wiley New York N.Y.

4. Bennett, P. M., J. Grinsted, and M. H. Richmond. 1977. Transposition of TnA does not generate deletions. Mol. Gen. Genet.154:205-211.

5. Clement, P., D. H. Pieper, and B. Gonzalez. 2001. Molecular characterization of a deletion/duplication rearrangement in tfd genes from Ralstonia eutropha JMP134(pJP4) that improves growth on 3-chlorobenzoic acid but abolishes growth on 2,4-dichlorophenoxyacetic acid. Microbiology147:2141-2148.

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