Affiliation:
1. Department of Animal Sciences, Colorado State University, Fort Collins, Colorado 80523
Abstract
ABSTRACT
Growth of
Escherichia coli
O157:H7 strain ATCC 43895 was monitored at 5, 10, 15, and 25°C in both pure and mixed (1:1) cultures with a gluconate-producing
Pseudomonas
sp. found in meat to evaluate the effect of the absence and presence of 1% glucose in broth on temperature-dependent competition. The number of colonies of the
Pseudomonas
strain exceeded 9 log CFU/ml under all conditions tested. The pathogen grew better as the temperature increased from 10 to 15 and 25°C and grew better in pure culture than in mixed cultures.
Pseudomonas
sp. inhibited
E. coli
O157:H7 in cocultures with glucose at 10°C, while at 15°C the pathogen exhibited a biphasic pattern of growth with an intermediate inactivation period. Pathogen inhibition was much weaker in cocultures grown without glucose at 10 to 15°C and, irrespective of glucose, at 25°C. These results indicate that glucose enhances the growth inhibition of
E. coli
O157:H7 by some
Pseudomonas
spp., potentially due to its rapid uptake and conversion to gluconate, at low (≤15°C) temperatures.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
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