Enzymatic Degradation of Chlorodiamino- s -Triazine-Reference-Cited by-同舟云学术

Enzymatic Degradation of Chlorodiamino- s -Triazine

Published:2002-09 Issue:9 Volume:68 Page:4672-4675
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Seffernick Jennifer L.¹²,Shapir Nir³⁴,Schoeb Michael³,Johnson Gilbert¹,Sadowsky Michael J.³²⁴,Wackett Lawrence P.¹³²

Affiliation:

1. Department of Biochemistry, Molecular Biology, and Biophysics

2. Center for Microbial and Plant Genomics

3. Biotechnology Institute

4. Department of Soil, Water, and Climate, University of Minnesota, St. Paul, Minnesota 55108

Abstract

ABSTRACT 2-Chloro-4,6-diamino- s -triazine (CAAT) is a metabolite of atrazine biodegradation in soils. Atrazine chlorohydrolase (AtzA) catalyzes the dechlorination of atrazine but is unreactive with CAAT. In this study, melamine deaminase (TriA), which is 98% identical to AtzA, catalyzed deamination of CAAT to produce 2-chloro-4-amino-6-hydroxy- s -triazine (CAOT). CAOT underwent dechlorination via hydroxyatrazine ethylaminohydrolase (AtzB) to yield ammelide. This represents a newly discovered dechlorination reaction for AtzB. Ammelide was subsequently hydrolyzed by N -isopropylammelide isopropylaminohydrolase to produce cyanuric acid, a compound metabolized by a variety of soil bacteria.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.9.4672-4675.2002

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