Improvement of the Immunochromatographic NG-Test Carba 5 Assay for the Detection of IMP Variants Previously Undetected-Reference-Cited by-同舟云学术

Improvement of the Immunochromatographic NG-Test Carba 5 Assay for the Detection of IMP Variants Previously Undetected

Published:2019-12-20 Issue:1 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Volland Hervé¹,Girlich Delphine²³,Laguide Marine²³,Gonzalez Camille²³,Paris Virginie²³,Laroche Maxime⁴,Oueslati Saoussen²³,Dortet Laurent²³⁵⁶^ORCID,Simon Stéphanie¹,Naas Thierry²³⁵⁶^ORCID

Affiliation:

1. Service de Pharmacologie et Immunoanalyse (SPI), CEA, INRA, Laboratoire d’Etudes et de Recherches en Immunonalyse, Université Paris-Saclay, Gif-sur-Yvette, France

2. EA7361 “Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases,” Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France

3. Evolution and Ecology of Resistance to Antibiotics Unit, Institut Pasteur–APHP, Université Paris Sud, Paris, France

4. NG Biotech, Z.A Courbouton, Guipry, France

5. Associated French National Reference Center for Antibiotic Resistance: Carbapenemase-producing Enterobacteriaceae, Le Kremlin-Bicêtre, France

6. Hygiene Unit, Assistance Publique/Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France

Abstract

Here, we evaluated the immunochromatographic assay NG-Test Carba 5v2 (NG-Biotech), with improved IMP variant detection on 31 IMP producers, representing the different branches of the IMP phylogeny, including 32 OXA-48, 19 KPC, 12 VIM, 14 NDM, and 13 multiple carbapenemase producers (CPs), 13 CPs that were not targeted, and 13 carbapenemase-negative isolates. All tested IMP variants were accurately detected without impairing detection of the other carbapenemases.

Funder

Assistance Publique Hôpitaux de Paris

Agence Nationale de la Recherche

Université Paris-Sud

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.01940-19

Reference24 articles.

1. Epidemiology and Mechanisms of Resistance of Extensively Drug Resistant Gram-Negative Bacteria