Automatic Identification of Individual rpoB Gene Mutations Responsible for Rifampin Resistance in Mycobacterium tuberculosis by Use of Melting Temperature Signatures Generated by the Xpert MTB/RIF Ultra Assay-Reference-Cited by-同舟云学术

Automatic Identification of Individual rpoB Gene Mutations Responsible for Rifampin Resistance in Mycobacterium tuberculosis by Use of Melting Temperature Signatures Generated by the Xpert MTB/RIF Ultra Assay

Published:2019-12-23 Issue:1 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cao Yuan¹,Parmar Heta¹,Simmons Ann Marie²,Kale Devika²,Tong Kristy²,Lieu Deanna²,Persing David²,Kwiatkowski Robert²,Alland David¹,Chakravorty Soumitesh¹²

Affiliation:

1. Department of Medicine, Rutgers New Jersey Medical School, Newark, New Jersey, USA

2. Cepheid Inc., Sunnyvale, California, USA

Abstract

Molecular surveillance of rifampin-resistant Mycobacterium tuberculosis can help to monitor the transmission of the disease. The Xpert MTB/RIF Ultra assay detects mutations in the rifampin resistance-determining region (RRDR) of the rpoB gene by the use of melting temperature ( T m ) information from 4 rpoB probes which can fall in one of the 9 different assay-specified T m windows.

Funder

HHS | National Institutes of Health

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00907-19

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