Evaluation of Diagnostic Tests for Clostridium difficile Infection-Reference-Cited by-同舟云学术

Evaluation of Diagnostic Tests for Clostridium difficile Infection

Published:2010-02 Issue:2 Volume:48 Page:606-608
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Swindells Jonathan¹,Brenwald Nigel¹,Reading Nathan¹,Oppenheim Beryl¹

Affiliation:

1. Department of Medical Microbiology, City Hospital, Birmingham, United Kingdom

Abstract

ABSTRACT We evaluated toxigenic Clostridium difficile detection by a lateral flow assay for antigen and toxin, an enzyme immunoassay, and two commercial PCR methods. Compared to the cell cytotoxicity neutralization assay and toxigenic culture, both toxin detection methods lacked sensitivity. PCR following combined antigen and toxin detection provided the most useful diagnostic information.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01579-09

Reference17 articles.

1. Barbut, F., M. Delmée, J. F. Brazier, J. C. Petit, I. R. Poxton, M. Rupnik, V. Lalande, C. Schneider, P. Mastrantonio, R. Alonso, E. Kuipjer, M. Tvede, and the ESCMID Study Group on Clostridium difficile. 2003. A European survey of diagnostic methods and testing protocols for Clostridium difficile. Clin. Microbiol. Infect. 9 : 989-996.

2. Delmée, M., J. Van Broeck, A. Simon, M. Janssens, and V. Avesani. 2005. Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for culture. J. Med. Microbiol. 54 : 187-191.

3. Rapid and Reliable Diagnostic Algorithm for Detection of Clostridium difficile

4. Abstr. 18th Eur. Congr. Clin. Microbiol. Infect. Dis. 2008

5. Is a Two-Step Glutamate Dehyrogenase Antigen-Cytotoxicity Neutralization Assay Algorithm Superior to the Premier Toxin A and B Enzyme Immunoassay for Laboratory Detection of Clostridium difficile ?

Cited by 138 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. C. difficile biomarkers, pathogenicity and detection;Clinica Chimica Acta;2024-05

2. A study to assess the prevalence and risk factors for Clostridioides difficile infection in patients with inflammatory bowel disease in a tertiary care hospital in Northern India;Journal of Laboratory Physicians;2024-03-27

3. Molecular methods for healthcare-acquired infections;Diagnostic Molecular Pathology;2024

4. Diagnosis of Clostridium difficile: a wrong algorithm and an improvement in immunoassays;2023-09-11

5. Overview of current detection methods and microRNA potential in Clostridioides difficile infection screening;World Journal of Gastroenterology;2023-06-14