Completed genomes for <i>Rickettsia rickettsii</i> isolated from ticks and quality controlled for motility phenotype-Reference-Cited by-同舟云学术

Completed genomes for Rickettsia rickettsii isolated from ticks and quality controlled for motility phenotype

Published:2023-10-19 Issue:10 Volume:12 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Nock Adam M.¹,Clark Tina R.¹,Barbian Kent²,Martens Craig²,Hackstadt Ted¹^ORCID

Affiliation:

1. Host-Parasite Interactions Section, Laboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, USA

2. Genomics Research Section, Research Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, Montana, USA

Abstract

ABSTRACT Complete genomes of Rickettsia rickettsii were sequenced with Illumina and PacBio technologies from low-passage isolates from ticks. These isolates were quality controlled for intact roaM , a regulator of actin-based motility that is negatively selected for in culture. The Sheila Smith strain was re-sequenced using the same methodology.

Funder

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00362-23

Reference15 articles.

1. Regulator of Actin-Based Motility (RoaM) Downregulates Actin Tail Formation by Rickettsia rickettsii and Is Negatively Selected in Mammalian Cell Culture

2. Canu: scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation

3. Cutadapt removes adapter sequences from high-throughput sequencing reads

4. Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement

5. Reactivity of monoclonal antibodies to Rickettsia rickettsii with spotted fever and typhus group rickettsiae