Comparison of rates of virus isolation from leukocyte populations separated from blood by conventional and Ficoll-Paque/Macrodex methods

Author:

Howell C L,Miller M J,Martin W J

Abstract

One hundred fifty-two blood specimens, largely from immunocompromised patients, were collected in heparinized Vacutainer tubes and divided into paired aliquots of equal volume. Buffy-coat preparations, containing mixed leukocyte and separate mononuclear and polymorphonuclear leukocyte populations were obtained by treatment of blood with conventional and Ficoll-Paque/Macrodex (F-P/M) methods. The development of cytopathic effect in monolayers of WI-38 fibroblasts inoculated with cell suspensions derived from the two methods was used to assess virus infectivity. Twice as many virus isolations were obtained using F-P/M. Of those viruses isolated by both conventional and F-P/M, the development of cytopathic effect was more extensive using the latter method. Moreover, a greater variety of viruses was isolated using F-P/M method, as compared to the conventional method. The F-P/M method is no more time consuming than conventional procedures, is readily adaptable for use in the diagnostic virology laboratory, requires only minimal additional cost, and is a particularly suitable and effective means of monitoring viremia.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference20 articles.

1. Asymptomatic cytomegalovirus infection in children with leukemia;Armstrong D.;Am. J. Dis. Child.,1971

2. Viral infections in renal transplant recipients;Armstrong J. A.;Infect. Immun.,1976

3. Separation of leukocytes from blood and bone marrow;Boyum A.;Scand. J. Clin. Lab. Invest.,1968

4. The role of a protective and prophylactic granulocyte transfusion in marrow transplantation;Buckner C.;Transplant. Proc.,1978

5. Cytomegaloviremia in children with acute Iymphocytic leukemia;Cox F.;J. Pediatr.,1975

Cited by 66 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Specimen Collection and Processing;Clinical Microbiology Procedures Handbook;2016-01-27

2. Detection of Hepatitis C virus RNA in peripheral blood mononuclear cells of patients with abnormal alanine transaminase in Ahvaz;Indian Journal of Medical Microbiology;2014-07

3. Viruses and Chlamydiae;Clinical Microbiology Procedures Handbook;2010-08

4. VIRAL LABORATORY DIAGNOSIS;Feigin and Cherry's Textbook of Pediatric Infectious Diseases;2009

5. Human Cytomegalovirus Tropism for Endothelial Cells: Not All Endothelial Cells Are Created Equal;Journal of Virology;2007-03

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3