Auto-MuRCiS: a streamlined software package for analysis of multiplex, randomized CRISPR interference sequencing data-Reference-Cited by-同舟云学术

Auto-MuRCiS: a streamlined software package for analysis of multiplex, randomized CRISPR interference sequencing data

Published:2024-09-10 Issue:9 Volume:13 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Myers Kevin S.¹^ORCID,Place Michael¹,Gasch Audrey P.¹²,Noguera Daniel R.¹³^ORCID,Donohue Timothy J.¹⁴^ORCID

Affiliation:

1. Wisconsin Energy Institute and Great Lakes Bioenergy Research Center, University of Wisconsin-Madison, Madison, Wisconsin, USA

2. Laboratory of Genetics and Center for Genomic Science Innovation, University of Wisconsin-Madison, Madison, Wisconsin, USA

3. Department of Civil and Environmental Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA

4. Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin, USA

Abstract

ABSTRACT Multiplex, randomized CRISPR interference sequencing (MuRCiS) allows for the simultaneous identification of multiple gene knockouts that together influence microbial processes. Here, we report on an updated analysis tool called Auto-MuRCiS that utilizes Docker to make the analysis of these data rapid and more user-friendly.

Publisher

American Society for Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/mra.00356-24

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