Simple and Rational Approach to the Identification of Mycobacterium tuberculosis , Mycobacterium avium Complex Species, and Other Commonly Isolated Mycobacteria-Reference-Cited by-同舟云学术

Simple and Rational Approach to the Identification of Mycobacterium tuberculosis , Mycobacterium avium Complex Species, and Other Commonly Isolated Mycobacteria

Published:2001-10 Issue:10 Volume:39 Page:3768-3771
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Wong Derek A.¹,Yip Peter C. W.¹,Cheung Danny T. L.²,Kam Kai Man¹

Affiliation:

1. Tuberculosis Reference Laboratory1 and

2. Public Health Laboratory, Kowloon Hospital,2 Pathology Service, Department of Health, HKSAR Government, Hong Kong

Abstract

ABSTRACT A novel PCR-restriction fragment length polymorphism analysis of the hsp65 gene was developed. The restriction patterns for Mycobacterium tuberculosis and Mycobacterium avium complex (MAC) species were designed to be highly distinct, and the overall number of restriction patterns was designed to be limited. Four hundred specimens (17 reference strains and 383 clinical isolates) were tested, of which 98 were M. tuberculosis and 132 were MAC species. The assay was virtually 100% sensitive and specific for M. tuberculosis and MAC species. Moreover, it gave highly concordant results for other mycobacterial species other than M. terrae complex species. This assay can be completed in one day and is user-friendly and robust. Therefore, it is highly suitable for large-scale use in a clinical laboratory.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.10.3768-3771.2001

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