Serotype-Specific Detection of Coxsackievirus A16 in Clinical Specimens by Reverse Transcription-Nested PCR

Author:

Bendig Justin W. A.1,O'Brien Patrick S.1,Muir Peter2

Affiliation:

1. PHLS Coxsackievirus Reference Unit, Epsom, Surrey,1 and

2. Department of Infection, Guy's, King's, and St. Thomas' School of Medicine, King's College London, London,2 United Kingdom

Abstract

ABSTRACT We describe the development of a coxsackievirus A16 (CVA16) serotype-specific PCR which correctly differentiated between CVA16 and other enterovirus serotypes of both laboratory isolates and clinical specimens. The assay will be useful for monitoring CVA16 outbreaks and studying the disease association, epidemiology, and evolution of this common enterovirus serotype.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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