Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination-Reference-Cited by-同舟云学术

Extraction and Genotyping of Cryptosporidium parvum DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination

Published:2001-01 Issue:1 Volume:39 Page:401-403
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Amar C.¹²,Pedraza-Dı́az S.¹,McLauchlin J.¹

Affiliation:

1. Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT,1 and

2. School of Chemical and Life Sciences, University of Greenwich, Woolwich, London, SE18 8PF,2 United Kingdom

Abstract

ABSTRACT A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.1.401-403.2001

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