Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes-Reference-Cited by-同舟云学术

Rapid Detection of Enterohemorrhagic Escherichia coli by Real-Time PCR with Fluorescent Hybridization Probes

Published:2001-01 Issue:1 Volume:39 Page:370-374
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bellin Tobias¹,Pulz Matthias²,Matussek Andreas¹,Hempen Hans-Günther¹,Gunzer Florian¹

Affiliation:

1. Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover,1 and

2. Niedersächisches Landesgesundheitsamt, 30449 Hannover,2 Germany

Abstract

ABSTRACT In this report, we present a PCR protocol for rapid identification of enterohemorrhagic Escherichia coli on a LightCycler instrument. In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.39.1.370-374.2001

Reference18 articles.

1. Polymerase chain reaction for diagnosis of enterohemorrhagic Escherichia coli infection and hemolytic-uremic syndrome

2. Escherichia coli O157:H7 and its significance in foods;Doyle M. P.;Int. J. Food Microbiol.,1991

3. Detection of Shiga-Like Toxin ( stx 1 and stx 2 ), Intimin ( eaeA ), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA ) Genes in Animal Feces by Multiplex PCR

5. Illnesses Associated withEscherichia coli0157:H7 Infections

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