Affiliation:
1. Microbiology Research Unit, Department of Oral Medicine and Oral Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland,1 and
2. Wadsworth Center, New York State Department of Health, Albany, New York2
Abstract
ABSTRACT
The methods currently available for the identification of the pathogenic yeast
Candida dubliniensis
all have disadvantages in that they are time-consuming, expensive, and/or, in some cases, unreliable. In a recent study (P. Staib and J. Morschhäuser, Mycoses 42:521–524; 1999) of 14
C. dubliniensis
and 11
C. albicans
isolates, it was suggested that the ability of
C. dubliniensis
to produce rough colonies and chlamydospores (chlamydoconidia) on Staib agar (SA) provided a simple means of differentiating it from its close relative
C. albicans
. In the present investigation, we examined the colony morphology and chlamydospore production of 130
C. dubliniensis
and 166
C. albicans
isolates on SA and on the related defined medium caffeic acid-ferric citrate agar (CAF). All of the
C. dubliniensis
and
C. albicans
isolates produced chlamydospores on the control medium, i.e., rice-agar-Tween agar. However, while none of the
C. albicans
isolates produced chlamydospores on either SA or CAF, 85.4 and 83.8% of the
C. dubliniensis
isolates produced chlamydospores on SA and CAF, respectively. All of the
C. albicans
isolates grew as smooth, shiny colonies on SA after 48 to 72 h of incubation at 30°C, while 97.7% of the
C. dubliniensis
isolates grew as rough colonies, many (65%) with a hyphal fringe. In contrast, 87.4% of the
C. albicans
and 93.8% of the
C. dubliniensis
isolates yielded rough colonies on CAF. Although the results of this study confirm that SA is a good medium for distinguishing between
C. dubliniensis
and
C. albicans
, we believe that discrimination between these two species is best achieved on the basis of colony morphology rather than chlamydospore production.
Publisher
American Society for Microbiology
Cited by
65 articles.
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