Molecular Characterization of First Human Bartonella Strain Isolated in Italy

Author:

Ciervo Alessandra1,Petrucca Andrea1,Ciarrocchi Simonetta1,Pinto Antonella1,Bonazzi Lucio2,Fabio Anna2,Farnetti Enrico2,Chomel Bruno B.3,Ciceroni Lorenzo1

Affiliation:

1. Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome,1 and

2. Department of Infectious Diseases, Microbiology and Molecular Biology, Arcispedale S. Maria Nuova, 42100 Reggio Emilia,2 Italy, and

3. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, California 956163

Abstract

ABSTRACT The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oklahoma, which was used as a control strain.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Bartonellaceae;Molecular Typing in Bacterial Infections, Volume II;2022

2. Global HIV and dermatology;Sande's HIV/AIDS Medicine;2012

3. Bartonella infections in Italy;Clinical Microbiology and Infection;2009-12

4. Optimization of pulse-field gel electrophoresis for Bartonella subtyping;Journal of Microbiological Methods;2009-01

5. Global HIV and Dermatology;Global HIV/AIDS Medicine;2008

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