Chi18A, the Endochitinase in the Cellulosome of the Thermophilic, Cellulolytic Bacterium Clostridium thermocellum-Reference-Cited by-同舟云学术

Chi18A, the Endochitinase in the Cellulosome of the Thermophilic, Cellulolytic Bacterium Clostridium thermocellum

Published:2002-06 Issue:6 Volume:68 Page:3176-3179
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Zverlov Vladimir V.¹,Fuchs Klaus-Peter²,Schwarz Wolfgang H.²

Affiliation:

1. Institute for Molecular Genetics, Russian Academy of Science, 123 187 Moscow, Russia

2. Research Group for Microbial Biotechnology, Technische Universität München, 85350 Freising, Germany

Abstract

ABSTRACT The chitinase gene chiA was identified on the Clostridium thermocellum genome downstream of the endoglucanase gene celA . It contains a catalytic module of glycosyl hydrolase family 18 and a cellulosomal dockerin module. Chi18A hydrolyzes aryl-acetyl-chito-oligosaccharides preferentially. In denaturing electrophoresis of purified cellulosomes, a single chitinase activity band was identified in zymograms and Western blots, indicating that Chi18A is the only chitinase in the cellulosome.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.68.6.3176-3179.2002

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