Affiliation:
1. Department of Microbiology, Institute of Biology, University of Hohenheim, Stuttgart, Germany
Abstract
C-tail proteins are anchored in the inner membrane with a transmembrane segment at the C terminus in an N-in/C-out topology. Due to this topology, membrane insertion occurs only posttranslationally. Nevertheless, the C-tail-anchored protein SciP is targeted cotranslationally by SRP. We report here that two amino-terminal hydrophobic stretches in SciP are individually recognized by SRP and target the nascent protein to FtsY. The presence of two signal sequences may enable a retargeting mechanism, as already observed for multispanning membrane proteins, to make the posttranslational insertion of SciP by YidC more efficient.
Funder
Deutsche Forschungsgemeinschaft
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
2 articles.
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