Author:
Honda T,Taga S,Takeda T,Hasibuan M A,Takeda Y,Miwatani T
Abstract
Lethal toxin was purified extensively from the culture filtrate of a Kanagawa phenomenon-positive strain of Vibrio parahaemolyticus. The purified toxin was a protein, and its homogeneity was demonstrated by sodium dodecyl sulfate-polyacrylamide gel disc electrophoresis and analytical ultracentrifugation. It was demonstrated that the thermostable direct hemolysin was identical to the lethal toxin and that it was the main, if not only, lethal toxin in the culture filtrate. The purified toxin had a lethal effect when injected into mice either intravenously or intraperitoneally. Its lethal effect was very rapid, a dose of 5 mug of toxin per mouse killing the animals within 1 min. The lethal activity was inhibited by a ganglioside mixture. Some physicochemical properties of the purified toxin are reported.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference23 articles.
1. Akehashi H. and M. Yoneda. 1967. Toxins of Vibrio parahaemolyticus (in Japanese) p. 215-231. In T. Fujino and H. Fukumi (ed.) Vibrio parahaemolyticus II. Naya Shoten Co. Tokyo.
2. Estimation of the molecular weight of proteins by Sephadex gel filtration;Andrews P.;Biochem. J.,1964
3. Microdetermination of inorganic phosphate, phospholipids, and total phosphate in biological materials;Baginski E. S.;Clin. Chem.,1967
4. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane;Fairbanks G.;Biochemistry,1971
5. Cholera;Finkelstein R. A.;CRC Crit. Rev. Microbiol.,1973
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