Cross-Priming Amplification for Rapid Detection of Mycobacterium tuberculosis in Sputum Specimens-Reference-Cited by-同舟云学术

Cross-Priming Amplification for Rapid Detection of Mycobacterium tuberculosis in Sputum Specimens

Published:2009-03 Issue:3 Volume:47 Page:845-847
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Fang Rendong¹²,Li Xia²,Hu Lin³,You Qimin³,Li Jing¹,Wu Jie¹,Xu Peng¹²,Zhong Huayan³,Luo Ying³,Mei Jian¹,Gao Qian²

Affiliation:

1. Department of TB Control, Shanghai Municipal Centers for Disease Control and Prevention, Shanghai 200336, China

2. Key Laboratory of Medical Molecular Virology, Institutes of Biomedical Sciences and Institute of Medical Microbiology, Fudan University, Shanghai 200032, China

3. Ustar Biotechnologies (Hangzhou) Co., Ltd., Hangzhou, Zhejiang 310012, China

Abstract

Cross-priming amplification (CPA) technology for tuberculosis diagnosis from sputum specimens was evaluated. The sensitivity of CPA from smear- and liquid culture-positive specimens was 96.9%, and that from smear-negative and liquid culture-positive specimens was 87.5%. The specificity of CPA in culture-negative specimens was 98.8%.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01528-08

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