Evaluation of Calcium-Enhanced Media for Colistin Susceptibility Testing by Gradient Agar Diffusion and Broth Microdilution

Author:

Green Daniel A.12ORCID,Macesic Nenad3,Uhlemann Anne-Catrin3,Lopez Mabel2,Stump Stephania3,Whittier Susan12,Schuetz Audrey N.4,Simner Patricia J.5,Humphries Romney M.67

Affiliation:

1. Department of Pathology and Cell Biology, Columbia University Irving Medical Center, New York, New York, USA

2. Clinical Microbiology Laboratory, NewYork-Presbyterian Hospital/Columbia University Irving Medical Center, New York, New York, USA

3. Division of Infectious Diseases, Columbia University Medical Irving Medical Center, New York, New York, USA

4. Division of Clinical Microbiology, Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA

5. Division of Medical Microbiology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

6. Accelerate Diagnostics, Tucson, Arizona, USA

7. Department of Pathology, University of Arizona, Tucson, Arizona, USA

Abstract

Despite the increasing reliance on polymyxin antibiotics (polymyxin B and colistin) for treatment of multidrug-resistant Gram-negative infections, many clinical laboratories are unable to perform susceptibility testing due to the lack of accurate and reliable methods. Although gradient agar diffusion is commonly performed for other antimicrobials, its use for polymyxins is discouraged due to poor performance characteristics.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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