Correlation between Viral Resistance to Zidovudine and Resistance at the Reverse Transcriptase Level for a Panel of Human Immunodeficiency Virus Type 1 Mutants-Reference-Cited by-同舟云学术

Correlation between Viral Resistance to Zidovudine and Resistance at the Reverse Transcriptase Level for a Panel of Human Immunodeficiency Virus Type 1 Mutants

Published:2001-08 Issue:15 Volume:75 Page:7202-7205
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Lennerstrand Johan¹,Hertogs Kurt²,Stammers David K.³,Larder Brendan A.¹

Affiliation:

1. Virco UK, Ltd., Cambridge CB4 0GA,1 and

2. Virco N.V., B-2800 Mechelen, Belgium2

3. Structural Biology Division, The Wellcome Trust Centre for Human Genetics, University of Oxford, OX3 7BN Oxford,3 United Kingdom, and

Abstract

ABSTRACT Using a large panel of human immunodeficiency virus type 1 site-directed mutants, we have observed a higher correlation than has previously been demonstrated between zidovudine (AZT)-triphosphate resistance data at the reverse transcriptase (RT) level and corresponding viral AZT resistance. This enhanced-resistance effect at the RT level was seen with ATP and to a lesser extent with PP i when ATP was added at physiological concentrations. The ATP-dependent mechanism (analogous to pyrophosphorolysis) appears to be dominant in the mutants bearing the D67N and K70R or 69 insertion mutations, whereas the Q151M mutation seems independent of ATP for decreased binding to AZT-triphosphate.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.75.15.7202-7205.2001

Reference26 articles.

1. Phenotypic mechanism of HIV-1 resistance to 3′-azido-3′-deoxythymidine (AZT): increased polymerization processivity and enhanced sensitivity to pyrophosphate of the mutant viral reverse transcriptase;Arion D.;Biochemistry,1998

2. Luminescent immobilized enzyme test systems for inorganic pyrophosphate: assays using firefly luciferase and nicotinamide-mononucleotide adenylyl transferase or adenosine-5′-triphosphate sulfurylase;Barshop B. A.;Anal. Biochem.,1991

3. HIV Population Dynamics in Vivo: Implications for Genetic Variation, Pathogenesis, and Therapy

4. A sensitive assay for the quantification of reverse transcriptase activity based on the use of carrier-bound template and non-radioactive-product detection, with special reference to human-immunodeficiency-virus isolation;Ekstrand D. H.;Biotechnol. Appl. Biochem.,1996

5. Infection of HTLV-III/LAV in HTLV-I-carrying cells MT-2 and MT-4 and application in a plaque assay;Harada S.;Science,1985

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