Affiliation:
1. Department of Genetics and Microbiology, University of Geneva Medical School, CMU, CH-1211 Geneva 4, Switzerland
Abstract
ABSTRACT
The “rule of six” stipulates that the
Paramyxovirus
RNA polymerase efficiently replicates only viral genomes counting 6
n
+ 0 nucleotides. Because the nucleocapsid proteins (N) interact with 6 nucleotides, an exact nucleotide-N match at the RNA 3′-OH end (3′-OH congruence) may be required for recognition of an active replication promoter. Alternatively, assuming that the six positions for the interaction of N with the nucleotides are not equivalent, the nucleotide position relative to N may be critical (N phase context). The replication abilities of various minireplicons, designed so that the 3′-OH congruence could be discriminated from the N phase context, were studied. The results strongly suggest that the application of the rule of six depends on the recognition of nucleotides positioned in the proper N phase context.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
57 articles.
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