Application of Targeted Next-Generation Sequencing Assay on a Portable Sequencing Platform for Culture-Free Detection of Drug-Resistant Tuberculosis from Clinical Samples

Author:

Cabibbe Andrea M.1,Spitaleri Andrea12,Battaglia Simone1,Colman Rebecca E.34,Suresh Anita3,Uplekar Swapna3,Rodwell Timothy C.34,Cirillo Daniela M.1ORCID

Affiliation:

1. Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Milan, Italy

2. Center for Omics Sciences, IRCCS San Raffaele Scientific Institute, Milan, Italy

3. Foundation for Innovative New Diagnostics, Campus Biotech, Geneva, Switzerland

4. Department of Medicine, University of California, San Diego, California, USA

Abstract

Targeted next-generation sequencing (tNGS) has emerged as a comprehensive alternative to existing methods for drug susceptibility testing (DST) of Mycobacterium tuberculosis from patient sputum samples for clinical diagnosis of drug-resistant tuberculosis (DR-TB). However, the complexity of sequencing platforms has limited their uptake in low-resource settings. The goal of this study was to evaluate the use of the tNGS-based DST solution Genoscreen Deeplex Myc-TB, for use on the compact, low-cost Oxford Nanopore Technologies MinION sequencer.

Funder

Foundation For Innovative New Diagnostics

Unitaid

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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