Impact of Competitive Inhibition and Sequence Variation upon the Sensitivity of Malaria PCR-Reference-Cited by-同舟云学术

Impact of Competitive Inhibition and Sequence Variation upon the Sensitivity of Malaria PCR

Published:2007-05 Issue:5 Volume:45 Page:1621-1623
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Bialasiewicz Seweryn¹²,Whiley David M.¹²,Nissen Michael D.¹²³,Sloots Theo P.¹²³

Affiliation:

1. Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Royal Children's Hospital and Health Service District, Herston, Queensland, Australia

2. Clinical Medical Virology Centre, University of Queensland, Brisbane, Queensland, Australia

3. Microbiology Division, Queensland Health Pathology Service, Royal Brisbane Hospital Campus, Brisbane, Queensland, Australia

Abstract

ABSTRACT Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02145-06

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