Evaluation of nonradioactive DNA probes for identification of mycobacteria-Reference-Cited by-同舟云学术

Evaluation of nonradioactive DNA probes for identification of mycobacteria

Published:1992-09 Issue:9 Volume:30 Page:2476-2478
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Lebrun L¹,Espinasse F¹,Poveda J D¹,Vincent-Levy-Frebault V¹

Affiliation:

1. Service de Microbiologie, Hôpital Antoine Béclère, Clamart, France.

Abstract

Commercial chemiluminescent DNA probes (Accuprobe; Gen-Probe, San Diego, Calif.) for the identification of Mycobacterium tuberculosis (MTB) complex, M. avium complex (MAC), M. gordonae, and M. kansasii were evaluated with 134 clinical isolates. These included 36 MTB complex, 40 MAC, 27 M. gordonae, 9 M. kansasii, and 22 Mycobacterium spp. The specificity was 100% for the four probes. The sensitivity was 100% for the MTB complex and M. gordonae probes and 95.2% for the MAC probe. Five of the nine M. kansasii isolates tested were not detected with the probe.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.30.9.2476-2478.1992

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