Affiliation:
1. Department of Microbiology, The Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, North Carolina 27103
Abstract
One of the crossovers leading to the transduction of
Escherichia coli
W3350 or one of its derivatives for the Gal
+
marker by T1 grown on donors lysogenic for λ must occur to the right of the galactose operon. The location of this crossover determined both the rate of transduction and the λ genes which control the transduction by (λ-
gal
)T1. When the crossover occurred either to the left of gene N or the right of the cI gene, it was affected in a positive fashion by the gene product of the N
+
gene. When the crossover occurred either to the left of gene N or between N and gene Q, its efficiency was not significantly affected by the expression of Q
+
. However, crossovers to the right of gene Q were greatly stimulated by the presence of a functioning Q gene on the chromosome of (λ-
gal
)T1 hybrid. The repressor made by the cI
+
gene of λ blocked efficient transduction. Therefore, the control of efficient transduction by (λ-
gal
)T1 is the same as the control of transcription of λ. It is concluded that the increased efficiency of transduction by (λ-
gal
)T1 in nonimmune recipients is not caused by any particular product of a gene but rather by the process of transcription itself.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
16 articles.
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