Affiliation:
1. Fermentation Research Laboratories, Sankyo Company Ltd., Shinagawa-ku, Tokyo, Japan
2. Institute for Physical and Chemical Research, Saitama, Japan
Abstract
The antibiotic polyoxin D was shown to inhibit the incorporation of
14
C-glucosamine into cell wall chitin in
Neurospora crassa
at levels which were comparable with those required for inhibition of fungal growth. At the same time, the antibiotic increased the accumulation of a nucleotide, which was identified as uridine diphosphate (UDP)-
N
-acetylglucosamine, indicating inhibition of chitin synthesis. Chitin synthetase (UDP-
N
-acetylglucosamine: chitin
N
-acetylglucosaminyl transferase, EC 2.4.1.16) of
N. crassa
was found to be strongly inhibited by polyoxin D, as determined by the transfer of
14
C-
N
-acetylglucosamine from
14
C-UDP-
N
-acetylglucosamine to the particulate fraction. The inhibition was competitive with respect to UDP-
N
-acetylglucosamine and specific for chitin synthetase. The
K
i
for polyoxin D in the reaction was 1.40 × 10
−6
m
, and the
K
m
for UDP-
N
-acetylglucosamine was 1.43 × 10
−3
m
. The formation of osmotically sensitive, protoplast-like structures, when the fungus
Cochliobolus miyabeanus
was grown in the presence of polyoxin D, also suggested that the primary site of action of polyoxin D was in the formation of cell wall structures.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
180 articles.
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