Affiliation:
1. Research Institute for Food Science, Kyoto University, Kyoto, Japan
Abstract
Crithidia
factors and folic acid were found to be widely distributed in culture fluids and in cells of 27 species of bacteria, when cultured under aerobic conditions into the stationary phase. Most bacteria excreted more
Crithidia
factors and folic acid than they retained in their cells. One
Crithidia
factor produced by
Serratia indica
and one produced by
Bacillus cereus
differed from biopterin in their chromatographic behavior. The factor excreted by
S. indica
appeared to be a 2-amino-4-hydroxy-6-substituted pteridine on the basis of KMnO
4
oxidation and ultraviolet absorption spectra. One of the folate compounds excreted by this organism was shown to be identical to 5,10-methylidynetetrahydrofolic acid by bioautography.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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